PSA: Protein Sequence Analyzer
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   Welcome to PSA (Protein Sequence Analyzer)
  To report bug, Email: ghosh.biplab@gmail.com
         Last modified on : 05-12-2021
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> Reading the input file for PSA

> Input options:
	calculation type = msa
	output directory = ./public/Outputs/1d90e0d2bdc355c6/
	input file = ./Inputs/1d90e0d2bdc355c6/inp.fasta
	log file = psa.log
	Hydrophobicity = yes
	ProtParam = yes
	CalculateMutualInformation = yes
	similarity threshold = 1
	pseudo count = 0

> Reading multiple sequence alignment data from: ./Inputs/1d90e0d2bdc355c6/inp.fasta
> Number of sequences present the input file = 200
> Number of sequences selected after applying filter = 200
> Now printing results in files
> Printing amino acid composition
> Printing residue frequency
> Printing atomic composition
> Printing total number of charged residues
> Printing total number of polar residues
> Printing total number of hydrophobic residues
> Printing total number of aliphatic residues
> Printing total number of aromatic residues
> Printing total number of tiny residues
> Printing total charge of the sequences
> Printing molecular weights
> Printing calculate pI
> Printing all the sequences with pI below 7.4
> Printing all the sequences with pI above 7.4
> Printing extinction coefficient
> Printing instability index
> Printing all the sequences which are stable
> Printing all the sequences which are unstable
> Printing aliphatic index
> Printing GRAVY values
> Printing the number of dipeptides
> Printing hydrophobicitymkdir: created directory './public/Outputs/1d90e0d2bdc355c6//Hydrophobicity'

> Printing in ProtParam formatmkdir: created directory './public/Outputs/1d90e0d2bdc355c6//ProtParam'

> Effective number of sequences, Meff =   200.00
> Printing probability of occurence of amino acids at a position
> Printing the most frequent residue for each position
> Printing the consensus sequence
> Printing the top conserved residues
> Printing poitional entropy
> Printing top poitional entropies
> Printing probability of occurence of amino acids at selected positions
> Printing residue frequency in the consensus sequence
> Printing the mutual information
> Printed all the results

> Total computation time is : 42.00 seconds

> Thank you for using PSA

  Program terminated